DETAILS, FICTION AND 2-FDCK HCL

The lab suggests fixation will trigger the dyes to leak in the cells. I do think the assumption is the fact that fixation could destruction cell membranes. I did find a paper which describes fixation employing methanol or acetic acid, right after staining using a DCFDA derivative. DCFDA is the reagent that stains ROS inside the kit ab113851, and I